Your guide to how to perform a subcutaneous injection Vitality Health SFL

09 May. 2025

How to Perform a Subcutaneous Injection for TRT, NAD+, or Vitamin Therapies

If you’re new to subcutaneous (sub-Q) injections, you might feel unsure about where to begin and a little uneasy about injecting. That’s okay! With the right techniques and proper preparation, you’ll find the process simple and manageable.

This guide walks you through step-by-step instructions for administering sub-Q injections for Testosterone Replacement Therapy (TRT), GLP-1s, NAD+, Amino Acids, HGH, or vitamin therapies safely and effectively.

What is a Subcutaneous Injection?

A subcutaneous injection involves delivering medication just under the skin into the fatty tissue. This method is common for therapies like TRT, NAD+, and vitamin supplementation. Sub-Q injections are typically less painful and easier to handle compared to deeper intramuscular injections.

Before You Begin

Gather your supplies!

  • Medication vial from Vitality Health (GLP-1s, TRT, NAD+, or vitamins)
  • Sterile syringe and needle
  • Alcohol swab
  • Sharps container for safe disposal of needles

Step-by-Step Instructions

1. Wash Your Hands

  • Thoroughly wash your hands with soap and water, scrubbing for at least 20 seconds.
  • Dry them with a clean towel or paper towels. Proper hand hygiene helps prevent infections.

2. Prepare Your Equipment

  • Check the medication vial for any discoloration, cloudiness, or particles. If it looks off, do not use it.
  • Remove the cap from the vial, if it’s new, and wipe the rubber stopper with an alcohol swab.

3. Draw the Medication

  • Attach the needle to the syringe if (it’s not preassembled). Most subQ needles are one piece.
  • Pull back the plunger to draw in the amount of air equal to your prescribed dose.
  • Insert the needle into the vial’s rubber stopper and push the air in. This helps create pressure inside the vial, making it easier to draw the medication.
  • Turn the vial upside down, ensuring the needle tip stays below the liquid level. Slowly pull back the plunger to fill the syringe with the correct dose.
  • Check for air bubbles in the syringe. If present, tap the syringe gently to bring them to the top, then push the plunger slightly to remove the air.

4. Choose the Injection Site

The most common sub-Q injection sites are:

  • The abdomen (avoiding the 2-inch area around your belly button)
  • The outer thigh
  • The back of the upper arm

Use a rotation system for injection sites to avoid irritation or tissue damage. If you’re unsure where to inject, consult your healthcare provider.

5. Clean the Injection Site

  • Use an alcohol swab to clean the skin at your selected site. Start at the center and use a circular motion, moving outward.
  • Allow the skin to air dry completely. Avoid blowing on it to speed up drying, as this can introduce bacteria.

6. Inject the Medication

  • Pinch a small fold of skin at the injection site with your non-dominant hand.
  • Hold the syringe like a dart with your dominant hand and insert the needle at a 45° to 90° angle (depending on how much fatty tissue is present).
  • Once the needle is in, slowly press the plunger to inject the medication. Take your time to avoid discomfort.

7. Remove the Needle

  • Once the medication is injected, remove the needle at the same angle it was inserted.
  • If there’s a small amount of bleeding, lightly press a clean gauze or cotton ball on the site. Avoid rubbing the area.

8. Dispose of the Needle and Syringe

  • Immediately place the used needle and syringe into a designated sharps container. Do not throw them in the trash or recycle bin.
  • If you don’t have a sharps container, many pharmacies or healthcare providers can supply one.

9. Monitor for Reactions

  • Watch the injection site for redness, swelling, or pain. A small amount of soreness is normal, but if you notice significant irritation, contact your healthcare provider.

Safety Tips

  • Always use a new sterile needle and syringe for each injection.
  • Never reuse or share needles or syringes.
  • Store medications as directed, usually in a cool, dry place or refrigerator if required.
  • Keep out of reach of children.
  • Consult your doctor or pharmacist if you’re unsure about your dosage or technique.

You Did It!

Performing subcutaneous injections might seem challenging at first, but with regular practice, it will soon become part of your self-care routine.

Call Vitality Health of South Florida at 1-833-4LOWTEE Or contact us through the website and get started.  Don’t be dissuaded by the name – we offer telehealth appointments in any of MD licensed states as well as in our office here in Florida.

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FDA Disclaimer: These products are not intended to diagnose, treat, cure, or prevent any disease.
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